To create and maintain sterile environment during the basic preprocess of microorganism (purebred) culture fermentation is the most important. To avoid bacteria interfere and affect the harvest rate, furthermore cause failure or variation (especially the secondary products of metabolism). To make a provision of sterile technical interpretation for equipment concern as follows: Ⅰ. No Blind Angle Design: Arc-shaped design no angle of all the wetted part inside the fermentor(fermenter, bioreacter, bioreactor), all the ports from the nearest stop valve or blind cover (neck) must be <2d (inner diameter). All the pipes with secondary SIP are required concept of sterilization buffer room, ex. sampling valve(tube), feeding tube, transfer tube, seal…etc. All wetted parts adopt diaphragm valve or least food or pharmaceutical sanitary valve (Note: still need to take care of the sterilization method.)。
Ⅱ. Complete Sterilization:
Ⅲ. Maintain Positive Pressure First enter cooling section during sterilization, relief pressure too fast will easily cause sudden boiling and thus should adopt segmented relief cooling.
And cooling too quickly, there will be negative pressure doubt in parts, especially the exhaust spot. It can install exhaust filter to prevent the pollution of the adverse pressure. But it should be noted that the exhaust filter get wet easily then clogged. It shall retrofit the electric heating piece in the filter housing to avoid it.
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